6th International conference on Infectious Diseases & Antibiotics August 28-29,2019 Paris, France, HOLIDAY INN PARIS - PORTE DE CLICHY

Yumn Ahmed Elsabagh is currently working as a Lecturer in the Department of Internal Medicine at Cairo University, Egypt. She has a MBBCH, a master’s degree and studied MD in Internal Medicine at Cairo University. She is a member of Egyptian Society of Rheumatology. She is involved in teaching and lectures on Tropical and Infectious diseases for Medical students. She has supervised many undergraduate and postgraduate researchers. She attended different conferences and published researches regarding infectious diseases in rheumatological patients.

Brucellosis is a zoonotic infection which still remains as a major health problem in the Mediterranean region and the Middle East. Two case reports were described. Case (1): A 40 years old female, known rheumatic heart disease with mitral valve replacement 23 years ago. She complained of fever for one year, arthritis of wrists, knees, ankles and low back pain. Four months later, she complained of left upper abdominal pain. By examination temperature was 40°C, looks pale, cardiac examination showed mixed mitral valve disease, abdomen showed tender splenomegaly and joint examination showed tenderness at wrists, knees, ankles and lumber spine. ESR 120, CRP: 96. TEE showed freely mobile mass attached to mitral valve. Blood culture showed MRSA and received antibiotics according to culture but without improvement. A presumptive diagnosis of Brucella Endocarditis was made. Brucella abortus: 1/640, Brucella mullitens: 1/320. We started streptomycin, doxycycline and rifampicin. After two weeks the patient clinical condition markedly improved, and after three months of treatment the vegetation resolved. Case (2): A female patient 20 years old, complained of diarrhea and fever reaching 40°C. After one week the patient complained of bilateral symmetrical arthritis of wrists, MCPs, PIPs, Knees and ankles with low back pain. Her lab showed HG 9, TLC: 19 with absolute neutrophilia. CRP: 98, ESR: 5, ANA, Anti DNA, Anti smith, Anti Jo, RF, Anti CCP, Anti SSA, Anti SSB, were negative. Blood culture showed Staphylococcus aureus. She received antibiotics according to culture without improvement. A presumptive diagnosis of Brucella arthritis was made. Brucella abortus: 1/640, Brucella mullitens: 1/640. We started doxycycline and rifampicin and the patient arthritis improved after two weeks.

Sonia Etenna Lekana-Douki is a Researcher, Co-Head of the Unit of Emergence of Viral Diseases in the Department of Virology, in the International Center of Medical Research of Franceville (CIRMF), Gabon. She has completed her DEA (Master) from Faculty of Chatenay-Malabry, University Paris-Sud (Paris XI), France in 2000. After working at a research engineer at CIRMF, she has completed her PhD from the University of Montpellier II in 2013, on the viral etiology of influenza-like illness among adults and children and diarrheal syndrome among children in Gabon. Her researches are focusing on diagnosis of infectious diseases and syndromic surveillance such as influenza-like illness, respiratory syndrome, diseases associated with arbovirus and diarrheal syndrome in children. She has published 13 papers, Head of the sequencing platform; she participates in various studies of the Department of Virology by genotyping the viruses diagnosed in the syndromic surveillance.

Background: Measles is one of the most infectious diseases with a high mortality rate worldwide. It is caused by the measles virus (MeV) which is a single stranded RNA virus with genetic diversity based on the nucleoprotein gene, including 24 genotypes. In Gabon, several outbreaks occurred in the past few years, especially in 2016 in Libreville and Oyem. A surveillance network of infectious diseases highlighted a measles outbreak which occurred in the south of Gabon from April to June 2017. Methods: Clinical specimens of urine, blood, throat and nasal swabs were collected in the two main cities of the Haut-Ogooue province, Franceville and Moanda. Virological investigations based on real-time polymerase chain reaction for molecular diagnosis and conventional PCR for genotype identification were done. Results: Specimens were collected from 139 suspected measles patients. A total of 46 (33.1%) children and adults were laboratory-confirmed cases among which 16 (34.8%) were unvaccinated, 16 (34.8%) had received one dose, and 11 (23.9%) had received two doses of the measles vaccine. Phylogenetic analysis revealed that all the sequences of the nucleoprotein gene belonged to genotype B3. Conclusions: Measles infection was more commonly confirmed among those with one recorded dose compared to suspect cases with none, unknown or two recorded doses. The molecular characterization of the strains showed the circulation of the B3 genotype which is endemic on the African continent, thirty years after the B2 genotype was described in an outbreak in Libreville, the capital of Gabon. These findings highlight that surveillance and molecular investigation of measles should be continued in Gabon